Use of N-(l-naphthyl)-ethylendiamine-dihydrochloride (NEDD) as secondary calibrator for conjugated bilirubin on the DuPont aca
نویسندگان
چکیده
منابع مشابه
Modification of the DuPont aca as exemplified by a new procedure for neonatal total bilirubin.
Hemoglobin interferes with determination of total bilirubin by the diazo procedure with the DuPont aca. We compared results by that procedure with those by a manual direct spectrophotometric two-wavelength method. Because the manual method was not inhibited by hemolysis as was the DuPont aca diazo procedure, we reprogrammed our DuPont aca to measure the absorbance of diluted serum samples at 45...
متن کاملNew kinetic procedure for the measurement of conjugated bilirubin with a centrifugal analyzer.
A procedure has been developed for the automated measurement of conjugated bilirubin in serum with a centrifugal analyzer. The conjugated bilirubin is measured by a fixed time kinetic method which monitors the reaction between conjugated bilirubin and diazotized sulfanilic acid at 550 nm. Results are calculated based on a comparison of the reagent blank-corrected absorbance changes between 15 s...
متن کاملDerivatives of 1-naphthyl ethylenediamine dihydrochloride for standardization of direct-bilirubin assays done with the Technicon "SMAC".
The Jendrassik--Groff assay for direct bilirubin was adapted for analysis rates of 150/h on the Technicon "SMAC" continuous-flow analyzer. This requires development of a standard that is compatible with the other 19 channels on this analyzer. N-1-Naphthyl ethylenediamine dihydrochloride has been used for standardization of direct bilirubin assays, but we found it to be unsuitable because values...
متن کاملA simple, solid-phase iodine-125 radioimmunoassay for theophylline.
The activity of y-glutamyltransferase (EC 2.3.2.2) is measured on the DuPont aca by an adaptation of the Szasz procedure ;-y-glutamyl-p-nitroanilide is transferred to glycylglycine, thereby releasing p-nitroaniline, which is measured at 405 nm. I have noticed low glutamyltransfer-ase activity in patients with cholestasis wherealkaline phosphate (EC 3.1.3.1) is increased, total bilirubin> 100 mg...
متن کاملPreincubation of serum aspartate aminotransferase with pyridoxal 5'-phosphate in the SMAC: comparison with revised DuPont aca method and recommended IFCC method.
The method for continuous-flow assay of aspartate aminotransferase with the Technicon SMAC was modified to include preincubation of the serum enzyme with pyridoxal 5'-phosphate, to be consistent with the recommendations of IFCC and the Standards Committee of AACC. Preliminary estimates of the imprecision of the modified method on SMAC gave day-to-day standard deviations of 5.3 U/L at mean of 48...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- The Journal of Automatic Chemistry
دوره 7 شماره
صفحات -
تاریخ انتشار 1985